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Catalog Centre de Ressources Biologiques de l'Institut Pasteur » CIP 102400 - Escherichia coli
Identity
Epidemiology
Collection group
Bacteria
Collection
CIP
Microorganism
Bactéries
Synonymous strain designation
ATCC 33694;DSM 1607;NCIMB 11865;CRBIP19.4
Incubation atmosphere
Aerobic
Incubation temperature
30
Cultivation time
24 H
Phenotype
Streptomycin resistant
Genotype
(F-) (l-) leuB6 proA2 recA13 thi-1 ara-14 lacY1 galK2 xyl-5 mtl-1 rpsL20 supE44 hsdS20 (rB- mB-)
Packaging
B
History
1986, Boyer, Paris, France: strain HB101
Properties
Hybrid K12 x B bacterium (mostly K12 though), containing the recA13 mutation that minimizes recombination and aids in insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogeneous restriction enzymes. Highly tranformable, suitable for vectors that do not require lacZ alpha-complementation.
Bibliography
J. Mol. Biol, 1969, 41, 459, doi:10.1016/0022-2836(69)90288-5; Methods Enzymol, 1979, 68, 245, doi:10.1016/0076-6879(79)68018-7
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